Ingredion has been granted a patent for methods of preparing steviol glycosides, including Rebaudioside D, E, M, N, and O. The patent also covers sweeteners and sweetened consumables containing these glycosides. The method involves passing an initial mixture of glycosides through a column packed with a polymer resin to collect a fraction with a high content of the desired glycosides. The resulting solution has a higher ratio of Reb DEMNO/T13SG compared to the initial mixture. GlobalData’s report on Ingredion gives a 360-degree view of the company including its patenting strategy. Buy the report here.
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According to GlobalData’s company profile on Ingredion, Nanoemulsion cosmetics was a key innovation area identified from patents. Ingredion's grant share as of September 2023 was 59%. Grant share is based on the ratio of number of grants to total number of patents.
Method for preparing steviol glycosides with high reb d, reb e, reb m, reb n, and reb o content
A recently granted patent (Publication Number: US11773125B2) discloses a method for preparing a steviol glycosides composition. Steviol glycosides are natural sweeteners derived from the Stevia rebaudiana plant. The method involves passing an initial mixture of steviol glycosides through a column packed with a polymer resin, where the steviol glycosides are weakly retained. The solvent is then used to collect a fraction with a high content of specific steviol glycosides, namely Reb D, Reb E, Reb M, Reb N, and Reb O, resulting in a solution with a higher Reb DEMNO/T13SG ratio compared to the initial mixture.
The initial mixture of steviol glycosides can include various sources such as purified Stevia rebaudiana plant material, commercially available Stevia extract, mixtures of steviol glycosides, by-products of other isolation and purification processes, synthetic or biosynthetic steviol glycosides, and more. The solvent used in the process can be pure water, aqueous acid, alcohol-water mixtures, or alcohol-aqueous acid mixtures with less than forty volume percent alcohol.
After collecting the fraction with a high content of specific steviol glycosides, the solvent can be removed to obtain a dry solid with a higher Reb DEMNO/T13SG ratio compared to the initial mixture. Additionally, the method can involve using alcohol-water or alcohol-aqueous acid mixtures with a higher alcohol volume percentage in one or more steps to collect a fraction with a low content of specific steviol glycosides, resulting in a fraction with a lower Reb DEMNO/T13SG ratio. The solvent can then be removed to obtain a dry solid or several dry solids with a lower Reb DEMNO/T13SG ratio compared to the initial mixture.
The patent also mentions the regeneration of the column packing solvent for subsequent loading of the initial mixture of steviol glycosides. The polymer resin used in the column can be a homopolymer or copolymer made from specific monomers, and it can have specific characteristics such as particle size and nitrogen mass content. The polymer resin can be made in the presence of polymerization initiators and may require washing to remove certain materials before usage.
Overall, this patent provides a method for preparing a steviol glycosides composition with specific ratios of different glycosides, allowing for the production of sweeteners with desired properties.
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