Nissui patented a method for producing microbial oil with increased EPA content by genetically modifying a labyrinthulid and collecting the oil. The labyrinthulid belongs to specific genera and has limited PUFA production activity. The method ensures microbial oil with at least 11.5% EPA content. GlobalData’s report on Nissui gives a 360-degree view of the company including its patenting strategy. Buy the report here.
According to GlobalData’s company profile on Nissui, Nanoemulsion cosmetics was a key innovation area identified from patents. Nissui's grant share as of February 2024 was 34%. Grant share is based on the ratio of number of grants to total number of patents.
Method for producing microbial oil with increased epa content
A recently granted patent (Publication Number: US11898190B2) discloses a method for producing microbial oil by genetically modifying a labyrinthulid, specifically by disrupting and/or silencing a fatty acid desaturase gene, culturing the modified labyrinthulid, and collecting the microbial oil produced. The labyrinthulid, belonging to the genus Parietichytrium or Schizochytrium, must have specific characteristics related to the synthesis of DHA and PUFAs via different pathways. The microbial oil obtained must meet specific composition criteria, with DHA and n-6 DPA not exceeding certain percentages of the total fatty acid composition.
The method described in the patent involves disrupting or silencing the fatty acid desaturase gene of the labyrinthulid using techniques such as electroporation, gene gun method, gene editing, antisense method, or RNA interference. Specific species within the genera Parietichytrium and Schizochytrium, such as Parietichytrium sarkarianum and Schizochytrium aggregatum, are mentioned as suitable candidates for this method. Additionally, particular strains like Parietichytrium sp. SEK358, Parietichytrium sarkarianum SEK364, Parietichytrium sp. SEK571, and Schizochytrium aggregatum ATCC 28209 are highlighted for their potential in producing microbial oil through this approach. This patent provides a detailed and specific method for enhancing microbial oil production by genetically modifying labyrinthulids with a focus on fatty acid desaturase genes and specific fatty acid composition requirements.
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