Carlsberg has patented a method using digital PCR to identify specific nucleotide mutations in mutated genes. The process involves mutagenizing a pool of organisms, dividing them into sub-pools, and amplifying target sequences to detect mutations. The method allows for precise identification of desired mutations in a large population of organisms. GlobalData’s report on Carlsberg gives a 360-degree view of the company including its patenting strategy. Buy the report here.

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According to GlobalData’s company profile on Carlsberg, Pest resistant transgenic crops was a key innovation area identified from patents. Carlsberg's grant share as of January 2024 was 49%. Grant share is based on the ratio of number of grants to total number of patents.

Method of identifying organisms with predetermined mutations in target sequences

Source: United States Patent and Trademark Office (USPTO). Credit: Carlsberg AS

A recently granted patent (Publication Number: US11884972B2) outlines a method for identifying organisms of a specific species carrying predetermined mutations in target nucleotide sequences. The method involves subjecting a pool of organisms to random mutagenesis, dividing the pool into sub-pools, preparing genomic DNA samples, performing PCR amplifications, and detecting amplification products with the predetermined mutations. By iteratively dividing and analyzing sub-pools, the method efficiently identifies organisms with the desired mutations. The patent specifies that the pool must contain at least 100,000 organisms, with each sub-pool consisting of at least 100 organisms with different genotypes.

Furthermore, the patent details variations of the method for different species, such as plants and unicellular organisms. For plants, the process includes steps like subjecting seeds to mutagenesis, growing them into mature plants, and dividing them into sub-pools for analysis. The method also allows for the identification of super-pools comprising mutations in multiple sub-pools. By utilizing compartmentalized PCR amplifications and droplets as spatially separated compartments, the method ensures accurate detection of mutations in the target sequences. Overall, the patent provides a comprehensive framework for efficiently identifying organisms with specific mutations, offering valuable insights for genetic research and breeding programs in various species.

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GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies across the world’s largest industries.