Amyris has patented a method for genetically engineering Kluyveromyces to produce and secrete antibodies. The method involves modifying the host cell genome, reducing NHEJ activity, and integrating donor DNA to enable secretion of the desired polypeptide. GlobalData’s report on Amyris gives a 360-degree view of the company including its patenting strategy. Buy the report here.
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According to GlobalData’s company profile on Amyris, Roll resistance tyre treads was a key innovation area identified from patents. Amyris's grant share as of January 2024 was 45%. Grant share is based on the ratio of number of grants to total number of patents.
Genetic engineering of kluyveromyces for antibody production
A recently granted patent (Publication Number: US11884928B2) discloses a method for modifying a target site in a Kluyveromyces host cell genome. The method involves reducing non-homologous end joining (NHEJ) activity in the host cell by integrating a nucleic acid at YKU70 or YKU80. Subsequently, the host cell is contacted with a nuclease capable of cleaving the target site and a donor DNA molecule encoding a polypeptide for homologous recombination. This results in the integration of the donor DNA at the target site. Additionally, the method includes contacting the host cell with a linear nucleic acid for homologous recombination, converting it into a circular extrachromosomal nucleic acid with a selectable marker coding sequence, and selecting a transformed host cell that secretes the encoded polypeptide.
Furthermore, the patent claims include variations such as the circular extrachromosomal nucleic acid containing a stability element from K. marxianus, a coding sequence for a guide RNA guiding the nuclease to the target site, and the use of an RNA-guided DNA endonuclease. The method also involves introducing a guide RNA for the RNA-guided DNA endonuclease on a plasmid with a stability element from K. marxianus and contacting the host cell with a linear nucleic acid encoding the guide RNA. These claims highlight the detailed process of genetic modification in Kluyveromyces host cells, offering potential applications in biotechnology and genetic engineering.
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GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies across the world’s largest industries.
