Amyris. has filed a patent for methods to efficiently produce isoprenoids using biosynthetic pathways, nucleic acids, enzymes, vectors, and genetically modified host cells. The patent also covers fermentation methods for high productivity. GlobalData’s report on Amyris gives a 360-degree view of the company including its patenting strategy. Buy the report here.
According to GlobalData’s company profile on Amyris, Roll resistance tyre treads was a key innovation area identified from patents. Amyris's grant share as of January 2024 was 45%. Grant share is based on the ratio of number of grants to total number of patents.
Robust production of isoprenoids using biosynthetic pathways
A recent patent application (Publication Number: US20240035061A1) discloses a method for producing a heterologous C5-C20 isoprenoid compound in a yeast host cell. The method involves obtaining yeast host cells capable of producing the compound, culturing them in specific media conditions, including glucose and ethanol, and recovering the compound from the fermentation mixture. The yeast host cells are engineered to express enzymes of the MEV or DXP pathway, resulting in the production of up to 40.0 grams of the compound per liter of fermentation mixture. The method showcases the potential for high yield production of farnesene, with specific claims stating production levels between 30 and 40 grams per liter.
Furthermore, the patent application outlines specific conditions for the cultivation of yeast host cells, including maintaining carbon-limited conditions, specific ethanol consumption rates, and phosphate concentrations in the media. The method also details the composition of the batch and feed media, with glucose and ethanol playing crucial roles in the production process. The use of S. cerevisiae as the yeast host cell and the expression of key enzymes in the pathway highlight the technical aspects of the method. Overall, the patent application provides a detailed and structured approach to producing heterologous C5-C20 isoprenoid compounds in yeast host cells, emphasizing the importance of specific media conditions and genetic modifications for achieving high production yields.
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